CCNT2 polyclonal antibody
产品名称： CCNT2 polyclonal antibody
英文名称： CCNT2 polyclonal antibody
产品编号： PAB11292
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against partial recombinant CCNT2.
- Immunogen:
- Recombinant protein corresponding to amino acids 1-584 of human CCNT2.
- Host:
- Rabbit
- Reactivity:
- Bovine, Dog, Human, Mouse, Rat
- Form:
- Liquid
- Storage Buffer:
- In antiserum (0.01% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Recombinant Protein.
- Recommend Usage:
- ELISA (1:5000-1:20000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Transcriptional activity and substrate recognition of cyclin T2 from P-TEFb.
Kurosu T, Zhang F, Peterlin BM.Gene. 2004 Dec 8;343(1):173-9.
- 2.
- Physical interaction between pRb and cdk9/cyclinT2 complex.
Simone C, Bagella L, Bellan C, Giordano A.Oncogene. 2002 Jun 13;21(26):4158-65.
- 3.
- 7SK small nuclear RNA binds to and inhibits the activity of CDK9/cyclin T complexes.
Nguyen VT, Kiss T, Michels AA, Bensaude O.Nature. 2001 Nov 15;414(6861):322-5.
- Applications
- Western Blot (Cell lysate)
- Western blot using CCNT2 polyclonal antibody (Cat # PAB11292) is shown to detect two major bands (arrowheads) corresponding to human CCN T2a and T2b as indicated.
Approximately 33 ug of a HeLa whole cell lysate was separated by 4-20% Tris Glycine SDS-PAGE.
After blocking the membrane with 5% BLOTTO in PBS, the membrane was probed forovernight at 4°C with the primary antibody diluted to 1 : 500 in 5% BLOTTO in PBS.
The membrane was washed and reacted with a 1 : 10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at room temperature (800 nm channel, green).
Molecular weight estimation was made by comparison to prestained MW markers indicated at the right (700 nm channel, red).
IRDye™ 800 fluorescenceimage was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
IRDye is atrademark of LI-COR, Inc.
- Entrez GeneID:
- 905
- Protein Accession#:
- NP_001232(isoforma);NP_490595(isoformb)
- Gene Name:
- CCNT2
- Gene Alias:
- FLJ90560,MGC134840
- Gene Description:
- cyclin T2
- Omim ID:
- 603862
- Gene Ontology:
- Hyperlink
- Gene Summary:
- The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin and its kinase partner CDK9 were found to be subunits of the transcription elongation factor p-TEFb. The p-TEFb complex containing this cyclin was reported to interact with, and act as a negative regulator of human immunodeficiency virus type 1 (HIV-1) Tat protein. Two alternatively spliced transcript variants, which encode distinct isoforms, have been described. [provided by RefSeq
- Other Designations:
- SDS-stable vimentin-bound DNA fragment HEF42VIM22,cyclin T2a,cyclin T2b,subunit of positive elongation transcription factor b
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