DUSP16 polyclonal antibody
产品名称： DUSP16 polyclonal antibody
英文名称： DUSP16 polyclonal antibody
产品编号： PAB4147
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of DUSP16.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human DUSP16.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Phosphorylation of Ser-446 determines stability of MKP-7.
Katagiri C, Masuda K, Urano T, Yamashita K, Araki Y, Kikuchi K, Shima H.J Biol Chem. 2005 Apr 15;280(15):14716-22. Epub 2005 Feb 2.
- 2.
- MAPK phosphatase DUSP16/MKP-7, a candidate tumor suppressor for chromosome region 12p12-13, reduces BCR-ABL-induced transformation.
Hoornaert I, Marynen P, Goris J, Sciot R, Baens M.Oncogene. 2003 Oct 30;22(49):7728-36.
- 3.
- Activation of ERK induces phosphorylation of MAPK phosphatase-7, a JNK specific phosphatase, at Ser-446.
Masuda K, Shima H, Katagiri C, Kikuchi K.J Biol Chem. 2003 Aug 22;278(34):32448-56. Epub 2003 Jun 6.
- Applications
- Western Blot (Cell lysate)
- Western blot analysis of DUSP16 polyclonal antibody (Cat # PAB4147) in 293 cell lysate (35 ug/lane). DUSP16 (arrow) was detected using the purified polyclonal antibody (1 : 50 dilution).
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with DUSP16 polyclonal antibody (Cat # PAB4147) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Entrez GeneID:
- 80824
- Protein Accession#:
- NP_085143;Q9BY84
- Gene Name:
- DUSP16
- Gene Alias:
- KIAA1700,MGC129701,MGC129702,MKP-7,MKP7
- Gene Description:
- dual specificity phosphatase 16
- Omim ID:
- 607175
- Gene Ontology:
- Hyperlink
- Gene Summary:
- The activation of mitogen-activated protein kinase (MAPK) cascades transduces various extracellular signals to the nucleus to induce gene expression, cell proliferation, differentiation, cell cycle arrest, and apoptosis. For full activation of MAPKs, dual-specificity kinases phosphorylate both threonine and tyrosine residues in MAPK TXY motifs. MKPs are dual-specificity phosphatases that dephosphorylate the TXY motif, thereby negatively regulating MAPK activity.[supplied by OMIM
- Other Designations:
- MAPK phosphatase-7
- Gene Pathway
- Related Disease