31985070 Opti-MEM
产品名称： 31985070 Opti-MEM
英文名称： Opti-MEM I Reduced Serum Medium
产品编号： 31985070
产品价格： 0
产品产地： 北京市通州区马驹桥联东U谷86A
品牌商标： Solarbio
更新时间： 2025-01-17T13:54:35
使用范围： null
- 联系人 : 索莱宝-龚思雨
- 地址 : 北京市通州区中关村科技园区通州园金桥科技产业基地景盛南四街15号85A三层
- 邮编 : 101102
- 所在区域 : 北京
- 电话 : 178****1073 点击查看
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- 邮箱 : 3193328036@qq.com
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北京索莱宝科技有限公司 张净花 010-56371206 13426459985
描述
Opti-MEM® Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change to growth rate or morphology. Opti-MEM® is also recommended for use with cationic lipid transfection reagents, such as Lipofectamine™ reagent. Opti-MEM® can be used with a variety of suspension and adherent mammalian cells, including Sp2, AE-1, CHO, BHK-21, HEK, and primary fibroblasts. Life Technologies offers a variety ofo® Opti-MEM® modifications for a range of cell culture applications.
This Opti-MEM® is modified as follows:
| With |
| • L-glutamine |
| • Phenol Red |
The complete formulation is confidential. For more information, please contact Technical Services.
Opti-MEM® is a unique media that contains insulin, transferrin, hypoxanthine, thymidine, and trace elements. These additional components allow for a reduction in serum supplementation by at least 50%.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
cGMP Manufacturing and Quality System
Opti-MEM® is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001 standards. For supply chain continuity, Life Technologiesoffers an identical ® Opti-MEM® product made in our Scotland facility (31985-047). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
® Opti-MEM® uses a sodium bicarbonate buffer system (2.4 g / L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
常见问题解答
This can occur if cells are overly trypsinized. Trypsinize for a shorter time or use less trypsin. Mycoplasma contamination could also cause this problem. Segregate your culture and test for mycoplasma infection. Lastly, check for attachment factors in the medium.
None. The term “reduced serum” means that one can reduce the amount of serum they use with classical cell culture media. When working with Opti-MEM™ I medium, most cells that are routinely cultured in serum-supplemented medium may be transferred directly into Opti-MEM™ I medium with a minimum of 50% reduction in serum.